【新进展】最新一期NATURE关于肿瘤干细胞研究的两篇PAPERS（4 January 2007）

【新进展】最新一期NATURE关于肿瘤干细胞研究的两篇PAPERS（4 January 2007）

肿瘤干细胞(cancer stem cells)作为一群具有无限增殖能力，导致恶性肿瘤复发的特殊肿瘤细胞吸引了越来越多研究人员的注意，最新一期NATURE（4 January 2007，olume 445 Number 7123 pp1-124）同时发表了加拿大和意大利两个研究小组独立的研究结果
“A human colon cancer cell capable of initiating tumour growth in immunodeficient mice Catherine A. O'Brien, Aaron Pollett, Steen Gallinger and John E. Dick”
“Identification and expansion of human colon-cancer-initiating cells Lucia Ricci-itiani1, Dario G. Lombardi2, Emanuela Pilozzi3, Mauro Biffoni1, Matilde Todaro4, Cesare Peschle1 and Ruggero De Maria1,2”。
两个研究小组的结果一致证实在人的结肠癌症细胞中，并不是所有的癌细胞都会在免疫缺陷小鼠身上新生出肿瘤，肿瘤干细胞只存在于CD133+的肿瘤细胞中，大约每262个CD133＋细胞中有一个肿瘤干细胞。其具有在NOD/SCID 小鼠模型上种植、生长出肿瘤并且继发转移的能力。这一研究结果提示：有效根治肿瘤的措施应当是针对肿瘤干细胞设计的。

A human colon cancer cell capable of initiating tumour growth in immunodeficient mice
Catherine A. O'Brien1, Aaron Pollett2, Steen Gallinger3 and John E. Dick1,4

Diision of Cell and Molecular Biology, Uniersity Health Network, Toronto, Ontario, M5G 1L7, Canada
Department of Pathology and Laboratory Medicine,
Center for Cancer Genetics-Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
Department of Molecular and Medical Genetics, Uniersity of Toronto, Toronto, Ontario, M5S 1A8, Canada
Correspondence to: John E. Dick1,4 Correspondence and requests for materials should be addressed to J.E.D. (Email: jdick@uhnres.utoronto.ca).

Top of pageColon cancer is one of the best-understood neoplasms from a genetic perspectie1, 2, 3, yet it remains the second most common cause of cancer-related death, indicating that some of its cancer cells are not eradicated by current therapies4, 5. What has yet to be established is whether eery colon cancer cell possesses the potential to initiate and sustain tumour growth, or whether the tumour is hierarchically organized so that only a subset of cells—cancer stem cells—possess such potential6, 7. Here we use renal capsule transplantation in immunodeficient NOD/SCID mice to identify a human colon cancer-initiating cell (CC-IC). Purification experiments established that all CC-ICs were CD133+; the CD133- cells that comprised the majority of the tumour were unable to initiate tumour growth. We calculated by limiting dilution analysis that there was one CC-IC in 5.7 104 unfractionated tumour cells, whereas there was one CC-IC in 262 CD133+ cells, representing >200-fold enrichment. CC-ICs within the CD133+ population were able to maintain themseles as well as differentiate and re-establish tumour heterogeneity upon serial transplantation. The identification of colon cancer stem cells that are distinct from the bulk tumour cells proides strong support for the hierarchical organization of human colon cancer, and their existence suggests that for therapeutic strategies to be effectie, they must target the cancer stem cells.

Identification and expansion of human colon-cancer-initiating cells
Lucia Ricci-itiani1, Dario G. Lombardi2, Emanuela Pilozzi3, Mauro Biffoni1, Matilde Todaro4, Cesare Peschle1 and Ruggero De Maria1,2

Department of Hematology and Oncology, Istituto Superiore di Sanità, iale Regina Elena 299, Rome 00161, Italy
Mediterranean Institute of Oncology, ia Penninazzo 7, iagrande 95029, Catania, Italy
Department of Laboratory Medicine and Pathology, Sant'Andrea Hospital, Uniersity 'La Sapienza', ia di Grottarossa 1037, Rome 00189, Italy
Department of Surgical and Oncological Sciences, Uniersity of Palermo, ia Liborio Giuffrè 5, Palermo 90127, Italy
Correspondence to: Ruggero De Maria1,2 Correspondence and requests for materials should be addressed to R.D.M. (Email: rdemaria@tin.it).

Top of pageColon carcinoma is the second most common cause of death from cancer1. The isolation and characterization of tumorigenic colon cancer cells may help to deise noel diagnostic and therapeutic procedures. Although there is increasing eidence that a rare population of undifferentiated cells is responsible for tumour formation and maintenance2, 3, 4, this has not been explored for colorectal cancer. Here, we show that tumorigenic cells in colon cancer are included in the high-density CD133+ population, which accounts for about 2.5% of the tumour cells. Subcutaneous injection of colon cancer CD133+ cells readily reproduced the original tumour in immunodeficient mice, whereas CD133- cells did not form tumours. Such tumours were serially transplanted for seeral generations, in each of which we obsered progressiely faster tumour growth without significant phenotypic alterations. Unlike CD133- cells, CD133+ colon cancer cells grew exponentially for more than one year in itro as undifferentiated tumour spheres in serum-free medium, maintaining the ability to engraft and reproduce the same morphological and antigenic pattern of the original tumour. We conclude that colorectal cancer is created and propagated by a small number of undifferentiated tumorigenic CD133+ cells, which should therefore be the target of future therapies.

能不能帖全文?谢谢好人

期待全文！

A human colon cancer cell capable of initiating tumour growth in immunodeficient mice

full text 1

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A human colon cancer cell capable of initiating tumour growth in immunodeficient mice

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还有一文献，哪位老大能够提供阿！！非常需要！！

gaihuan168 wrote:
还有一文献，哪位老大能够提供阿！！非常需要！！

楼上也是做结肠癌干细胞方面的吗

thanks a lot!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

大家都是做结肠癌的吗 ?期待与大家共同讨论

都在赶时髦啊，希望看到根本的突破，

癌干细胞!很热!希望大家看后写下你的思考!